4ASA
The structure of modified benzoquinone ansamycins bound to yeast N- terminal Hsp90
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-28 |
Detector | ADSC CCD |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 74.480, 74.480, 111.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.480 - 2.250 |
R-factor | 0.18205 |
Rwork | 0.179 |
R-free | 0.23452 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IN-HOUSE MODEL |
RMSD bond length | 0.019 |
RMSD bond angle | 2.218 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.480 | 2.310 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.050 | 0.640 |
Number of reflections | 15425 | |
<I/σ(I)> | 4.2 | 2.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 4.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |