4AQX
Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-01 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 45.839, 71.401, 179.054 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.793 - 2.200 |
R-factor | 0.2041 |
Rwork | 0.202 |
R-free | 0.24180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g9z |
RMSD bond length | 0.010 |
RMSD bond angle | 1.279 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.790 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.120 | 0.380 |
Number of reflections | 30701 | |
<I/σ(I)> | 7.1 | 1.9 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |