4AP5
Crystal structure of human POFUT2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 118.628, 118.628, 196.227 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.657 - 3.003 |
R-factor | 0.1767 |
Rwork | 0.173 |
R-free | 0.23590 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.275 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | autoSHARP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.120 | 0.400 |
Number of reflections | 31375 | |
<I/σ(I)> | 21 | 2.4 |
Completeness [%] | 96.4 | 71.6 |
Redundancy | 11.3 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 12% PEG 20000, 0.02 M TRIS PH 8.5 |