4AMJ
Turkey beta1 adrenergic receptor with stabilising mutations and bound biased agonist carvedilol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-27 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.056, 62.196, 100.926 |
Unit cell angles | 90.00, 109.17, 90.00 |
Refinement procedure
Resolution | 95.330 - 2.300 |
R-factor | 0.20354 |
Rwork | 0.202 |
R-free | 0.24005 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y02 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.394 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.100 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.100 | 0.590 |
Number of reflections | 47122 | |
<I/σ(I)> | 11.3 | 2.1 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 6.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 277 | 0.1 M BICINE PH 9.0, 22% PEG 600, 4 DEGREES CELSIUS. |