4AL8
Structure of Dengue virus DIII in complex with Fab 2H12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.401, 60.603, 62.833 |
| Unit cell angles | 93.32, 102.10, 101.51 |
Refinement procedure
| Resolution | 30.550 - 1.660 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.19750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 79666 | |
| <I/σ(I)> | 39.7 | 3.3 |
| Completeness [%] | 97.0 | 96 |
| Redundancy | 7.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
