4AKM
Crystal structure of the human lysosome-associated membrane protein LAMP-3 (aka DC-LAMP)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.10371, 1.1042, 1.1009 |
| Spacegroup name | P 31 |
| Unit cell lengths | 52.600, 52.600, 141.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.295 - 2.687 |
| R-factor | 0.2277 |
| Rwork | 0.227 |
| R-free | 0.24920 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.050 | 0.460 |
| Number of reflections | 11238 | |
| <I/σ(I)> | 15.48 | 2.47 |
| Completeness [%] | 91.1 | 97.9 |
| Redundancy | 2.86 | 2.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | PROTEIN WAS CRYSTALLIZED FROM 5% PEG 6000, 0.1 M CITRIC ACID, PH 5.0; THEN SOAKED IN 0.014 M AMMONIUM HEXACHLOROIRIDATE(III)HYDRATE AND CRYO-PROTECTED IN 30% PEG 6000. |
