4AK8
Structure of F241L mutant of langerin carbohydrate recognition domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-27 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 42 |
| Unit cell lengths | 79.959, 79.959, 90.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.520 - 1.400 |
| R-factor | 0.17754 |
| Rwork | 0.175 |
| R-free | 0.21859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c22 |
| RMSD bond length | 0.040 |
| RMSD bond angle | 3.020 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.520 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.330 |
| Number of reflections | 111133 | |
| <I/σ(I)> | 24.88 | 4.7 |
| Completeness [%] | 99.2 | 98 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | HANGING DROP METHOD 100 MM MES PH 6.0, 200 MM MGCL2 AND 17% PEG 3350 |
