4AK4
High resolution structure of Galactose Binding lectin from Champedak (CGB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.171, 121.729, 77.736 |
| Unit cell angles | 90.00, 90.61, 90.00 |
Refinement procedure
| Resolution | 24.160 - 1.650 |
| R-factor | 0.1661 |
| Rwork | 0.165 |
| R-free | 0.18980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ku8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 122.170 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.110 | 0.470 |
| Number of reflections | 169730 | |
| <I/σ(I)> | 13.4 | 1.9 |
| Completeness [%] | 96.5 | 80.2 |
| Redundancy | 3.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 40% PEG 600, 100 MM PHOSPHATE/CITRATE BUFFER PH 4.2 |
