4AIG
ADAMALYSIN II WITH PHOSPHONATE INHIBITOR
Experimental procedure
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 279 |
Detector technology | IMAGE PLATE AREA DETECTOR |
Collection date | 1996-11 |
Detector | MARRESEARCH |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 73.500, 73.500, 96.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.177 |
Rwork | 0.177 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iag |
RMSD bond length | 0.007 |
RMSD bond angle | 24.500 * |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.112 | 0.331 |
Total number of observations | 53112 * | |
Number of reflections | 19533 | |
<I/σ(I)> | 4 | 2 |
Completeness [%] | 95.4 | 98.3 |
Redundancy | 2.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8 * | 4 * | 1.8 M AMMONIUM SULPHATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | 4 (mM) | ||
3 | 1 | drop | 4 (mM) | ||
4 | 1 | drop | inhibitor | 0.8 (mg/ml) | |
5 | 1 | drop | Tris | 8 (mM) | |
6 | 1 | drop | ammonium sulfate | 0.36 (M) | |
7 | 1 | reservoir | ammonium sulfate | 1.8 (M) |