4AIF
AIP TPR domain in complex with human Hsp90 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-16 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.820, 104.490, 69.270 |
| Unit cell angles | 90.00, 97.41, 90.00 |
Refinement procedure
| Resolution | 40.405 - 2.006 |
| R-factor | 0.1903 |
| Rwork | 0.188 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TPR HOMOLOGY MODEL |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (FOR MR) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.410 | 8.970 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.060 | 0.590 |
| Number of reflections | 29974 | |
| <I/σ(I)> | 10.9 | 2.4 |
| Completeness [%] | 99.4 | 98.5 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1 M AMMONIUM SULFATE, 0.1 M BIS-TRIS PH 5.5, 1% PEG 3350 |
