4AFL
The crystal structure of the ING4 dimerization domain reveals the functional organization of the ING family of chromatin binding proteins.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-09 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 128.450, 186.600, 62.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.688 - 2.275 |
R-factor | 0.2073 |
Rwork | 0.205 |
R-free | 0.25600 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 0.995 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.090 | 2.400 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.090 | 0.360 |
Number of reflections | 34302 | |
<I/σ(I)> | 11.1 | 2.1 |
Completeness [%] | 96.8 | 96.8 |
Redundancy | 6.8 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 24% PEG 3350, 0.1 M BISTRIS- PROPANE PH:7.5, 0.4 M SODIUM NITRATE, 10% OPTISALT SOLUTION 6, SILVER BULLET ADDITIVE 3. |