4ADT
Crystal structure of plasmodial PLP synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 180.471, 180.471, 102.042 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.420 |
| R-factor | 0.18507 |
| Rwork | 0.183 |
| R-free | 0.23236 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nv2 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.678 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 24370 | |
| <I/σ(I)> | 21.6 | 3.1 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 6.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.5 |
