4AC9

CRYSTAL STRUCTURE OF TRANSLATION ELONGATION FACTOR SELB FROM METHANOCOCCUS MARIPALUDIS IN COMPLEX WITH GDP

Replaces:  1WB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-21
Detector	MARRESEARCH MAR165
Spacegroup name	P 31 1 2
Unit cell lengths	146.920, 146.920, 297.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.975 - 3.030
R-factor	0.1913
Rwork	0.190
R-free	0.22210
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.004
RMSD bond angle	0.978
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CNS
Refinement software	PHENIX ((PHENIX.REFINE: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.030	3.030
Rmerge	0.120	1.270
Number of reflections	139069
<I/σ(I)>	14.37	2
Completeness [%]	99.6	99.8
Redundancy	8	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				10 MM KCL,20 MM TRIS/HCL (PH 7.5),40 MM MGSO4,2 MM DTT,3 MM GDP,1.8 - 2.0 M AMMONIUM SULFATE,100 MM SODIUM CITRATE (PH 6.0),1 MM DEOXYCHOLIC ACID.