4AC3
S.pneumoniae GlmU in complex with an antibacterial inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2006-02-14 |
| Detector | RIGAKU CCD |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 117.090, 117.090, 116.619 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.100 |
| R-factor | 0.2347 |
| Rwork | 0.233 |
| R-free | 0.26968 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g97 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.036 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.300 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.290 |
| Number of reflections | 54029 | |
| <I/σ(I)> | 8.1 | 3.4 |
| Completeness [%] | 99.4 | 97.6 |
| Redundancy | 5.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 18-23% PEG 3350, 0.2M AMMONIUM SULPHATE, 25 MM TRIS-HCL, PH 7.5, 50MM NACL, 2MM TCEP |
