4AB3
ATP-triggered molecular mechanics of the chaperonin GroEL
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8.5 |
| Software | Flex-EM,NAMD,UCSF Chimera,IMAGIC,SPIDER |
| Symmetry | D7 |
