4AAA
Crystal structure of the human CDKL2 kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.819, 70.220, 83.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.780 - 1.530 |
| R-factor | 0.18178 |
| Rwork | 0.180 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3niz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.563 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.140 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.140 | 1.140 |
| Number of reflections | 53461 | |
| <I/σ(I)> | 13.4 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NACL, 0.1M TRIS PH 8.5; 25% PEG 3350. |
