4A9K
BROMODOMAIN OF HUMAN CREBBP WITH N-(4-hydroxyphenyl)acetamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-26 |
| Detector | RIGAKU SATURN A200 |
| Spacegroup name | H 3 |
| Unit cell lengths | 120.943, 120.943, 40.471 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.470 - 1.810 |
| R-factor | 0.16175 |
| Rwork | 0.160 |
| R-free | 0.19748 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.999 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.840 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.100 | 0.430 |
| Number of reflections | 19824 | |
| <I/σ(I)> | 14.5 | 3.3 |
| Completeness [%] | 98.2 | 99.2 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 150 MM KSCN, 20% PEG 3350, 4 DEGREES CELSIUS. |
