4A9F
N-TERMINAL BROMODOMAIN OF HUMAN BRD2 WITH 1-METHYLPYRROLIDIN-2-ONE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-04 |
| Detector | RIGAKU SATURN A200 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.232, 55.531, 67.975 |
| Unit cell angles | 90.00, 94.85, 90.00 |
Refinement procedure
| Resolution | 23.600 - 1.730 |
| R-factor | 0.17886 |
| Rwork | 0.177 |
| R-free | 0.21389 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.931 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.600 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.060 | 0.350 |
| Number of reflections | 42840 | |
| <I/σ(I)> | 9.8 | 2.3 |
| Completeness [%] | 98.2 | 95.6 |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 0.1M HEPES PH 7, 24-28% PEG 3350, 0.2 M (NH4)2SO4, 20 DEGREES CELSIUS |
