4A97
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with zopiclone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-02 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.400, 266.830, 110.760 |
Unit cell angles | 90.00, 109.20, 90.00 |
Refinement procedure
Resolution | 43.468 - 3.343 |
R-factor | 0.208 |
Rwork | 0.205 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vl0 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.755 |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.950 |
High resolution limit [Å] | 3.340 |
Rmerge | 0.110 |
Number of reflections | 83009 |
<I/σ(I)> | 12.8 |
Completeness [%] | 99.6 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |