4A90
Crystal structure of mouse SAP18 residues 1-143
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-12 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.420, 65.140, 61.610 |
| Unit cell angles | 90.00, 119.40, 90.00 |
Refinement procedure
| Resolution | 23.688 - 1.900 |
| R-factor | 0.2164 |
| Rwork | 0.215 |
| R-free | 0.25040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a6q |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.548 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.570 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 25741 | |
| <I/σ(I)> | 14.6 | 3.9 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M SODIUM CACODYLATE PH 6.5, 5% POLYETHYLENEGLYCOL 8000, 5% GLYCEROL, 40% 2-METHYL-2,4-PENTANEDIOL |
