4A8S
Non-Catalytic Ions Direct the RNA-Dependent RNA Polymerase of Bacterial dsRNA virus phi6 from De Novo Initiation to Elongation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.510, 91.710, 142.080 |
| Unit cell angles | 90.00, 102.05, 90.00 |
Refinement procedure
| Resolution | 33.490 - 2.900 |
| R-factor | 0.2179 |
| Rwork | 0.216 |
| R-free | 0.25020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hhs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.000 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.190 | |
| Number of reflections | 53974 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 99.0 | |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG 20000, 100 MM MES (PH 6.5) |
