4A7W
Crystal structure of uridylate kinase from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-21 |
Detector | ADSC CCD |
Spacegroup name | H 3 2 |
Unit cell lengths | 137.420, 137.420, 152.891 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.288 - 1.800 |
R-factor | 0.1903 |
Rwork | 0.188 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bnd |
RMSD bond length | 0.006 |
RMSD bond angle | 1.038 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.500 |
Number of reflections | 51462 | |
<I/σ(I)> | 37.3 | 4.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.1 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |