4A5T
STRUCTURAL BASIS FOR THE CONFORMATIONAL MODULATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 118.460, 118.460, 179.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.800 - 3.490 |
| R-factor | 0.2106 |
| Rwork | 0.208 |
| R-free | 0.25010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.150 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 3.580 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 0.270 | 0.980 |
| Number of reflections | 16059 | |
| <I/σ(I)> | 10.3 | 3 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 13.1 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
