4A4B
Structure of modified phosphoTyr371-c-Cbl-UbcH5B-ZAP-70 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 74.112, 74.112, 449.706 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.725 - 2.789 |
| R-factor | 0.1913 |
| Rwork | 0.187 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2CBL 2esk 1FBV. |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.940 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.090 | 0.620 |
| Number of reflections | 19621 | |
| <I/σ(I)> | 18.9 | 3.3 |
| Completeness [%] | 99.4 | 96.8 |
| Redundancy | 19 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 31-35% PENTAERYTHRITOL PROPOXYLATE (5/4 PO/OH), 0.2 M KCL, 50 MM HEPES, PH 7.5 |
