4A36
Structure of duck RIG-I helicase domain bound to 19-mer dsRNA and ATP transition state analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-05 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 41 |
Unit cell lengths | 129.270, 129.270, 106.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.950 - 3.700 |
R-factor | 0.19628 |
Rwork | 0.193 |
R-free | 0.26270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a2p |
RMSD bond length | 0.007 |
RMSD bond angle | 1.281 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.800 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.190 | 0.970 |
Number of reflections | 18825 | |
<I/σ(I)> | 10.8 | 2.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.55 | 7.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 12 MG/ML, 1.2 TIMES MOLAR RATIO OF 19-MER DSRNA, 4 MM ADP, 2 MM ALF3 AND 0.1 M HEPES PH 7.5, 5% (V/V) ISO-PROPANOL AND 10% (W/V) PEG 4000. |