4A2X
Structure of duck RIG-I C-terminal domain (CTD) with 14-mer dSRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-22 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 216.020, 90.640, 62.960 |
| Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
| Resolution | 108.010 - 4.000 |
| R-factor | 0.20603 |
| Rwork | 0.204 |
| R-free | 0.24813 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a2v |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.200 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.120 | 0.800 |
| Number of reflections | 10316 | |
| <I/σ(I)> | 10.3 | 1.9 |
| Completeness [%] | 98.4 | 94 |
| Redundancy | 4.47 | 4.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 14 MG/ML IN A 2:1 MOLAR RATIO WITH 14-MER DSRNA AND 0.1 M TRIS PH 8.8, 26% (W/V) PEG 3350. |
