4A2O
STRUCTURE OF THE HUMAN EOSINOPHIL CATIONIC PROTEIN IN COMPLEX WITH SULFATE ANIONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.264, 51.290, 55.394 |
| Unit cell angles | 90.00, 111.28, 90.00 |
Refinement procedure
| Resolution | 51.620 - 1.690 |
| R-factor | 0.16533 |
| Rwork | 0.162 |
| R-free | 0.22251 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dyt |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.941 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.620 | 1.780 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.070 | 0.450 |
| Number of reflections | 25341 | |
| <I/σ(I)> | 11.4 | 2.3 |
| Completeness [%] | 98.5 | 90.8 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.5 |
