Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-18 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.470, 86.210, 152.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.260 - 2.300 |
| R-factor | 0.1905 |
| Rwork | 0.189 |
| R-free | 0.22463 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gyc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.279 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.470 |
| Number of reflections | 34113 | |
| <I/σ(I)> | 1.5 | 1.6 |
| Completeness [%] | 97.1 | 87.1 |
| Redundancy | 6.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PEG 1000, PEG 8000, HEPES 100 MM, pH 7.0 |
