4A21
Structure of Mycobacterium tuberculosis fructose 1,6-bisphosphate aldolase bound to sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 336.945, 43.234, 103.020 |
Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
Resolution | 42.873 - 2.350 |
R-factor | 0.2151 |
Rwork | 0.211 |
R-free | 0.26100 |
Structure solution method | OTHER |
RMSD bond length | 0.006 |
RMSD bond angle | 0.984 |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.500 |
High resolution limit [Å] | 2.350 |
Rmerge | 0.110 |
Number of reflections | 71704 |
<I/σ(I)> | 3.8 |
Completeness [%] | 99.3 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% PEG 8000, 1.8 M LI2SO4, 50 MM TRIS/HOAC PH 7.5. |