4A1Y
Human myelin P2 protein, K65Q mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 43 |
| Unit cell lengths | 83.030, 83.030, 77.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.13145 |
| Rwork | 0.131 |
| R-free | 0.15382 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wut |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.371 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.040 | 0.660 |
| Number of reflections | 154219 | |
| <I/σ(I)> | 19.5 | 1.4 |
| Completeness [%] | 93.9 | 63.2 |
| Redundancy | 5.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
