4A1J

Ykud L,D-transpeptidase from B.subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-22
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 2
Unit cell lengths	56.240, 86.280, 74.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.115 - 2.200
R-factor	0.1974
Rwork	0.192
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y7m
RMSD bond length	0.008
RMSD bond angle	1.072
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.400	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.370
Number of reflections	18649
<I/σ(I)>	16.1	6.5
Completeness [%]	98.4	88.7
Redundancy	11.7	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				100 MM BIS-TRIS PH 5.5, 200 MM AMSO4, 25 % PEG 3350 AND 2 MM MURNAC MONOSACCHARIDE.