4A1G
The crystal structure of the human Bub1 TPR domain in complex with the KI motif of Knl1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-07 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.279, 130.969, 74.956 |
| Unit cell angles | 90.00, 110.17, 90.00 |
Refinement procedure
| Resolution | 61.983 - 2.600 |
| R-factor | 0.1889 |
| Rwork | 0.186 |
| R-free | 0.24360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3esl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.173 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 32541 | |
| <I/σ(I)> | 13.7 | 3.2 |
| Completeness [%] | 98.8 | 99.6 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1.25-1.3 M NA MALONATE, PH 6.0. |
