4A1G
The crystal structure of the human Bub1 TPR domain in complex with the KI motif of Knl1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-07 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.279, 130.969, 74.956 |
Unit cell angles | 90.00, 110.17, 90.00 |
Refinement procedure
Resolution | 61.983 - 2.600 |
R-factor | 0.1889 |
Rwork | 0.186 |
R-free | 0.24360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3esl |
RMSD bond length | 0.009 |
RMSD bond angle | 1.173 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.000 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.070 | 0.500 |
Number of reflections | 32541 | |
<I/σ(I)> | 13.7 | 3.2 |
Completeness [%] | 98.8 | 99.6 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 1.25-1.3 M NA MALONATE, PH 6.0. |