4A0Z
Structure of the global transcription regulator FapR from Staphylococcus aureus in complex with malonyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.380, 68.500, 61.160 |
| Unit cell angles | 90.00, 111.31, 90.00 |
Refinement procedure
| Resolution | 49.730 - 1.900 |
| R-factor | 0.19995 |
| Rwork | 0.198 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a0x |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.393 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.350 |
| Number of reflections | 30557 | |
| <I/σ(I)> | 16.4 | 3.6 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% MPD, 5% PEG-4000, 0.1M NA HEPES, PH=7.5 |
