4ZXB
Structure of the human insulin receptor ectodomain, IRDeltabeta construct, in complex with four Fab molecules
Replaces: 2DTGReplaces: 3LOHExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 121.870, 321.250, 199.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.460 - 3.300 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.23680 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.330 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BUSTER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 57953 | |
<I/σ(I)> | 11.39 | 0.78 |
Completeness [%] | 97.9 | 86.6 |
Redundancy | 7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Crystals were produced by vapour diffusion using 1 microlitre of the complex (2.6 mg/ml) in 10 mM HEPES (pH 7.5), 0.02% sodium azide and 10% D-trehalose solution and 1 microlitres of well solution containing 10% PEG 8000, 0.1 M MES (pH 6.5) and 0.1 M MgAc2. |