4ZWJ
Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2014-11-06 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.32 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.240, 109.240, 452.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.038 - 3.302 |
| R-factor | 0.2552 |
| Rwork | 0.253 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a4m 4j2q 3sn6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.021 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.500 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 62613 | |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 76.3 | 6.4 |
| Redundancy | 383.3 | 116.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 293 | PEG400, sodium acetate, magnesium acetate |
