4ZQR
Crystal Structure of the Catalytic Domain of the Inosine Monophosphate Dehydrogenase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.217, 75.233, 75.291 |
| Unit cell angles | 108.30, 108.25, 111.88 |
Refinement procedure
| Resolution | 35.726 - 1.692 |
| R-factor | 0.1528 |
| Rwork | 0.151 |
| R-free | 0.18280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.726 | 1.730 |
| High resolution limit [Å] | 1.692 | 1.700 |
| Rmerge | 0.330 | |
| Number of reflections | 129346 | |
| <I/σ(I)> | 17.2 | 2.6 |
| Completeness [%] | 91.4 | 51.5 |
| Redundancy | 2.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 289 | 0.1 M Na/K phosphate pH 6.2, 25 %(v/v) 1,2 propandiol, 10 %(v/v) glycerol |
