4ZLD
Crystal structure of human Roquin-2 ROQ domain in complex with Roquin CDE RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.236, 89.955, 84.632 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.470 - 1.600 |
R-factor | 0.15623 |
Rwork | 0.155 |
R-free | 0.17635 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.258 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.600 |
Number of reflections | 29045 |
<I/σ(I)> | 24.9 |
Completeness [%] | 95.9 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG8000, 100 mM Tris, 200 mM LiCl |