4ZJB

Crystal structure of (3R)-Hydroxyacyl-Acyl Carrier Protein Dehydratase(FabZ) in complex with holo-ACP from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	150
Detector technology	CCD
Collection date	2007-11-18
Detector	AGILENT ATLAS CCD
Wavelength(s)	1
Spacegroup name	P 2 3
Unit cell lengths	127.705, 127.705, 127.705
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.926 - 2.550
R-factor	0.1936
Rwork	0.190
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2GLL and 1L0I
RMSD bond length	0.010
RMSD bond angle	1.309
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.550
Number of reflections	22941
<I/σ(I)>	49.5
Completeness [%]	99.7
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			PEG 3350, sodium citrate