4ZIU
Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7 to the C-terminus.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763, 0.9788 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 86.720, 136.190, 172.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.980 - 2.700 |
R-factor | 0.1916 |
Rwork | 0.190 |
R-free | 0.23990 |
Structure solution method | SIRAS |
Starting model (for MR) | dataset of a Se-Met crystal collected at the selenium absorption peak and a native dataset to higher resolution |
RMSD bond length | 0.010 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.048 | 0.654 |
Number of reflections | 28350 | |
<I/σ(I)> | 24.3 | 2.9 |
Completeness [%] | 99.4 | 95.3 |
Redundancy | 6.4 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 20% [w/v] PEG4,000, 10% isopropanol 100mM Tris-HCl |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 20% [w/v] PEG4,000, 10% isopropanol 100mM Tris-HCl |