4ZI8

Structure of mouse clustered PcdhgC3 EC1-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	44.123, 82.286, 237.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.170 - 1.698
R-factor	0.2051
Rwork	0.204
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zi9
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.170	1.759
High resolution limit [Å]	1.698	1.698
Rmerge	0.112	1.079
Number of reflections	84132
<I/σ(I)>	5.38	0.53
Completeness [%]	86.0	39
Redundancy	3.3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	seeded from 100 mM HEPES pH 7, 60% MPD, 100 mM CaCl2, into 100 mM HEPES pH 7, 20% MPD