4ZHT
Crystal structure of UDP-GlcNAc 2-epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.041, 98.102, 154.722 |
| Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.690 |
| R-factor | 0.19061 |
| Rwork | 0.189 |
| R-free | 0.22509 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.640 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.690 |
| Number of reflections | 43536 |
| <I/σ(I)> | 14.8 |
| Completeness [%] | 99.8 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M ammonium acetate, 0.1 M HEPES, pH 7.1, 19% PEG 3350 |
