4ZHI
Eg5 motor domain mutant E162S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-10 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.720, 81.220, 82.600 |
Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.300 |
Rwork | 0.254 |
R-free | 0.30350 |
Data reduction software | SAINT |
Data scaling software | PROTEUM PLUS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.067 | 0.256 |
Number of reflections | 30404 | |
<I/σ(I)> | 8.45 | 2.77 |
Completeness [%] | 98.1 | 96.6 |
Redundancy | 1.85 | 1.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 200 mM NaF and 18-20% PEG 3350 |