4ZH4

Crystal structure of Escherichia coli RNA polymerase in complex with CBRP18

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.910
Spacegroup name	P 21 21 21
Unit cell lengths	185.581, 204.105, 308.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.200 - 3.993
R-factor	0.2504
Rwork	0.250
R-free	0.28290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lk1
RMSD bond length	0.005
RMSD bond angle	0.988
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.070
High resolution limit [Å]	4.000	10.820	4.000
Rmerge	0.176	0.106	0.998
Rmeas	0.179	0.110
Rpim	0.048	0.031	0.277
Total number of observations	1430622
Number of reflections	99049
<I/σ(I)>	6.2
Completeness [%]	100.0	99.8	100
Redundancy	14.4	13	13.8
CC(1/2)		0.985	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.1 M HEPES, 0.2 M calcium chloride, 18% PEG400