4ZFG

Dual-specificity Fab 5A12 in complex with Angiopoietin 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	181.668, 109.292, 43.946
Unit cell angles	90.00, 100.47, 90.00

Refinement procedure

Resolution	43.210 - 2.270
R-factor	0.1794
Rwork	0.177
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in-house ang2 and generic fab models
RMSD bond length	0.010
RMSD bond angle	1.130
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.350
High resolution limit [Å]	2.270	4.890	2.270
Rmerge	0.055	0.033	0.542
Number of reflections	38887
<I/σ(I)>	23.045
Completeness [%]	99.5	99.6	99.1
Redundancy	3.8	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.1 M Tris pH 8.5, 0.2 M lithium sulfate, 25% PEG 3350, 3% D(+)-sucrose