4ZDP
The crystal structure of Y334C mutant of human SepSecS in complex with selenocysteine tRNA (tRNASec)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-05-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 167.263, 167.263, 240.146 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.649 - 2.703 |
| R-factor | 0.2018 |
| Rwork | 0.200 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hl2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.056 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
| Rmerge | 0.113 | 0.031 | |
| Rmeas | 0.118 | 0.034 | |
| Rpim | 0.051 | 0.013 | 0.506 |
| Total number of observations | 599300 | ||
| Number of reflections | 98463 | ||
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 94.0 | 99.5 | 91.3 |
| Redundancy | 6.1 | 7 | 5.6 |
| CC(1/2) | 0.999 | 0.616 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.1M Na-cacodylate, pH 6.0 0.24M lithium citrate 8% (w/v) PEG 3,350 |
