4ZCR
Crystal structure of the C-terminal catalytic domain of Plasmodium falciparum CTP:phosphocholine cytidylyltransferase in complex with phosphocholine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.972957 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 50.620, 69.570, 117.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.510 - 1.800 |
R-factor | 0.17892 |
Rwork | 0.176 |
R-free | 0.23220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zct |
RMSD bond length | 0.009 |
RMSD bond angle | 1.218 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.510 |
High resolution limit [Å] | 1.800 |
Number of reflections | 18719 |
<I/σ(I)> | 14.3 |
Completeness [%] | 99.6 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG 4k |