4ZCM
Crystal Structure of Escherichia coli GTPase BipA/TypA Complexed with ppGpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.821, 160.125, 89.718 |
| Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
| Resolution | 48.471 - 3.310 |
| R-factor | 0.2354 |
| Rwork | 0.233 |
| R-free | 0.29110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zci |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.023 |
| Data reduction software | MOSFLM |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_1819) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.471 | 3.580 |
| High resolution limit [Å] | 3.310 | 3.310 |
| Rmerge | 0.170 | 0.700 |
| Number of reflections | 23655 | |
| <I/σ(I)> | 7 | 1.8 |
| Completeness [%] | 98.9 | 98.7 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris-HCl, 2 % PEG 6000, and 5 mM [Co(NH3)6]Cl3, pH 8.0 |
