4Z9V
TCTP contains a BH3-like domain, which instead of inhibiting, activates Bcl-xL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.357, 100.357, 105.045 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.535 - 2.099 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p1l |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.769 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1702) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.535 | 2.230 |
| High resolution limit [Å] | 2.099 | 2.099 |
| Rmerge | 0.074 | |
| Number of reflections | 31973 | |
| <I/σ(I)> | 19.2 | 1.1 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 12.7 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.5M Tris-sodium citrate, 100mM Pipes pH |
