4Z9S
Non-covalent assembly of monoubiquitin that mimics K11 poly-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-25 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.319, 81.301, 58.597 |
| Unit cell angles | 90.00, 129.33, 90.00 |
Refinement procedure
| Resolution | 24.570 - 2.300 |
| Rwork | 0.185 |
| R-free | 0.22295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.750 | |
| High resolution limit [Å] | 2.300 | |
| Rmerge | 0.047 | 0.147 |
| Number of reflections | 13167 | |
| <I/σ(I)> | 16.6 | 7.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 292 | 3.4M SODIUM MALONATE, 0.1 M SODIUM THIOSUCCINATE PH4.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 292K |
