4Z99
Crystal structure of the apo Low Molecular Weight Protein Tyrosine Phosphatase isoform A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-26 |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.667, 54.157, 100.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.750 - 2.300 |
R-factor | 0.19467 |
Rwork | 0.190 |
R-free | 0.28682 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5pnt |
RMSD bond length | 0.013 |
RMSD bond angle | 1.487 |
Data reduction software | APEX |
Data scaling software | APEX |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.160 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.226 | 0.621 |
Number of reflections | 8392 | |
<I/σ(I)> | 7.43 | 2 |
Completeness [%] | 99.7 | 100 |
Redundancy | 8.57 | 7.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 27% (w/v) PEG 2000, 0.1 M malic acid:Tris, molar ratio 1:2, pH 7.0 |